Figure 3.

In vivo MitoQ treatment reduces levels of reactive oxygen species (ROS) and neutrophil extracellular trap (NET) formation in neutrophils. Mice (eight mice per treatment group) were administered either drinking water alone, or water containing MitoQ (200 µM) for 11 weeks following weaning. Neutrophils were purified from bone marrow and assayed for (A) ROS production and (B) NET formation either spontaneously or following activation with A23187. Per cent control refers to the comparison of each value to the mean of female neutrophils with A23187 activation. Shown are points for individual mice and their mean for three experiments. Statistical analysis was unpaired t-test. ns, non-significant.