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. Author manuscript; available in PMC: 2020 Jun 9.
Published in final edited form as: Nat Microbiol. 2019 Dec 9;5(2):239–247. doi: 10.1038/s41564-019-0619-y

Extended Data Fig. 3. Multiple feeding events increase the potential of Aedes albopictus to transmit ZIKV.

Extended Data Fig. 3

(a) Schematic of experimental design. Paired bodies (MGI; midgut infection; % of mosquitoes with viral RNA in their bodies), legs (DI; disseminated infection; % of body positive mosquitoes with viral RNA in their legs) and salivary glands (SGI; salivary gland infection; % of leg positive mosquitoes with viral RNA in their salivary glands) were collected and assayed for the presence of viral RNA (b) 7 dpi and (c) 10 dpi. (d) SGI data from (b) and (c) analyzed as the % of ZIKV-exposed mosquitoes with a salivary gland infection. The data presented represents at least three experimental replicates. (●) single-feed; (●) double-feed. Data were analyzed by Two-sided Fisher’s exact test. (*) p<0.05, (**) p<0.01, (***) p<0.001. Sample sizes (represented as a fraction of positive samples/ total samples) for each treatment/ timepoint are embedded in the figures above each experimental group. Center values represent the proportion and error bars represent the binomial SE of sample proportions.