Table 2. Reported P-values of ANOVA and ANOSIM tests based on aitchison distances of microbial abundance.

A. Reported P-value
	anosim_aitchison	anova_aitchison
Control	0.112	0.072
Single-Mix	0.604	0.495
Multi-Mix	0.625	0.274
B. Reported P-value
	anosim_aitchison	anova_aitchison
TP1	0.218	0.165
TP2	0.001	0.001
TP3	0.007	0.02

The ANOSIM and ANOVA methods were both used to track any significant changes between each treatment group with regard to species abundance and composition over three different time points. The data were normalized using the Aitchison norm statistical analysis method. An alpha level of 0.01 was used for analysis.

A. Against this alpha level, no significant changes in abundance and/or composition were observed across any of the time points for all treatments in both the ANOSIM and ANOVA analyses.

B. For the first time point, there were no significant changes in species composition or abundance between the treatment groups. However, for both time points 2 and 3, significant changes in the microbial composition between treatment groups were supported by both the ANOSIM and ANOVA results which generated p-values below the given alpha-level value of 0.01. An exception was that at time point 3 only the ANOSIM generated a p-value lower than 0.01, but the p-value from ANOVA was also very small (p<0.05).