Fig 3. Grifolin, neogrifolin and confluentin inhibited cell viability in human cancer cells.

HeLa human cervical cancer cells and human colon cancer cells (SW480 and HT29) were treated with various concentrations of grifolin, neogrifolin and confluentin for 48 hours. The concentrations of compounds used were: 80, 60, 40, 20, 10, 5, 2.5, 1.25 and 0.625 μM. Cell viability was determined using the cytotoxic MTT assay. Each dose was tested in triplicate in each experiment and the data shown is a representation of at least three independent experiments (n = 3).