Skip to main content
. 2020 May 5;15(5):e0231948. doi: 10.1371/journal.pone.0231948

Fig 4. Effect of confluentin on apoptosis in SW480 human colon cancer cells.

Fig 4

(A) SW480 cells were treated with 2% DMSO or 50 μM confluentin for 24 hours after which cells were analyzed by flow cytometry to assess for necrosis (Q1), late apoptosis (Q2), early apoptosis (Q3) and live cells (Q4). Data shown represents three independent experiments (n = 3). (B) The percentages of cells in late apoptosis (Q2) treated with 2% DMSO or 50 μM confluentin from three independent experiments were combined and shown to be statistically significant (p < 0.05). (C) As in (A), SW480 cells treated with 2% DMSO or 50 μM confluentin for 24 hours followed by isolation of cell lysates. Western blot analyses were performed and data shown is a representative from five independent experiments (n = 5). (D) The level of Cleaved Caspase-3 in confluentin-treated cells was normalized to that of GAPDH and expressed relative to DMSO-treated cells (taken as 1.0). The plotted graph is from five independent experiments. For statistical analysis, a Student’s t-test was performed (p < 0.05). Error bars are S.D.