Fig 8. Binding curves of the full-length and truncated IMP1 to KRAS RNA as determined using fluorescence polarization.

Increasing amounts of the full-length IMP1 or truncated IMP1 (KH1to4 and KH3&4) were incubated with 44-nt fluorescein-labeled KRAS RNA at 37°C for 30 min. Results shown represent three replicate experiments (n = 3).