Fig 7. Conidial cell wall localization of BbAFP1.
(A) Representative images of conidia of the BbAFP1promoter::BbAFP1::eGFP strain counter stained with the membrane dye FM4-64. (B) Representative images of release of BbAFP1::eGFP from conidia by ultrasonic treatment (UT). Control experiments were performed using the constitutive expressing PgpdA::eGFP strain. (C) Detection of solubilized BbAFP1::eGFP via fluorometry in the supernatant of conidial suspensions (BbAFP1promoter::BbAFP1::eGFP) treated by ultrasonic treatment. Control experiments were performed using the wild type and constitutive expressing PgpdA::eGFP strains. (D) Western blot analyse of BbAFP1::eGFP in the conidial supernatant after ultrasonic treatment. Proteins were transferred to membrane and probes with an anti-GFP antibody. UT indicates ultrasonic treatment. All experiments were performed in triplicate with at least three independent biological samples. Error bars = SD, ** indicates P < 0.01, t-test.