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. 2020 Apr 23;16(4):e1008330. doi: 10.1371/journal.pgen.1008330

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Fig 5 — A) Cy-tRNA^SerUCA-mediated TMS assay in S. pombe yNB5 (tit1Δ) transformed with the empty plasmid pRep82X (sector 2) or plasmids expressing the proteins indicated (sectors 3–8), and the tit1⁺ control strain, yYH1 (sector 1). B) TMS assay as in A but displayed as dilution spot assay, for plasmids expressing TRIT1 and variants from pRep82X and from the stronger nmt1⁺ promoter (pRep4X) as indicated on the left. C) Western blot of extracts of cells in B detected with anti-TRIT1 (top panel), and anti-actin antibody; lanes numbers are indicated below actin panel.