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. 2020 Apr 23;16(4):e1008330. doi: 10.1371/journal.pgen.1008330

Fig 8. Evidence against a hyper-restriction model for TRIT1.

Fig 8

A) Results of PHA6 i6A37-sensitive PHA6 northern blot assay after expressing a human nuclear gene for tRNATyrGUA-G37-A38 with a G37A point mutant in mod5Δ MT-8 S. cerevisiae cells (lanes 2–5, 7) and with native G37 (lane 6), in the presence of empty vector (EV) or the IPTases indicated above the lanes (T32A = TRIT1-T32A); lanes 1 and 8 show ABL-8, the MOD5 positive strain. B) Body probe of the blot in A (upper panel), and EtBr stained gel (lower panel). C) Quantitation of % i6A37 modification of the cy-tRNATyrGUA from biological duplicate PHA6 northern blots as in panels A-B; error bars indicate standard deviation (SD).