Fig. 2.

Sinensetin inhibited influenza A virus-induced expression of cytokines and chemokines in A549 cells. a Influenza A virus carrying Gaussia luciferase reporter was used for determination of the antiviral effects of sinensetin. b-c A549 cells were infected with A/PR/8/34 (H1N1) (MOI = 0.1) with or without sinensetin treatment for 24 h. The mRNA and protein levels of IL-6, IP-10, TNF-α, IL-8 and MCP-1 was determined by qRT-PCR (b) and Luminex (c). Data are expressed as mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 compared to influenza virus-infected alone