Fig. 3.

Sinensetin inhibited influenza A virus-induced expression of COX-2 and PGE₂ in A549 cells. Influenza virus A/PR/8/34 (H1N1) (MOI = 0.1) infected A549 cells with or without indicated concentrations of sinensetin (30, 60, 120 μg/mL) treatment for 24 h. a-b COX-2 levels were detected by qRT-PCR (a) and immunoblot analysis (b), respectively. c COX-2 expression was quantified using the imageJ software. d PGE₂ production in the culture supernatants was measured by ELISA. Data are expressed as mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, *** P < 0.001 compared to influenza virus-infected alone