Fig. 2.

Closely related capsule biosynthesis gene clusters demonstrating cases of small genetic replacements. Genes are represented by arrows orientated in the direction of transcription that are coloured according to the scheme shown below. Shading between gene clusters indicates regions of >95 % nucleotide sequence similarity (dark grey) or 90–95 % nucleotide sequence similarity (light grey). Figure drawn to scale using the GenBank accession numbers listed in Table S1. (a) KL63 and KL108 gene clusters differing in wzy sequence. (b) KL1 and KL107 are examples of gne1 presence vs. absence. (c) KL13, KL73, KL12 and KL110 are examples of several closely related gene clusters with small sequence replacements altering the synthesis pathway of a complex sugar substrate, or topology of the CPS structure.