Figure 1. CIM6P/IGF2R is expressed in rat hippocampal neurons and mostly localizes to the somatic compartment.

(a) Immunofluorescence co-staining of CIM6P/IGF2R and GFAP, Iba1, or MAP2. Upper panels: representative composite tile scans of whole hippocampus (scale bar, 500 μm). Lower panels: CA1, CA2, CA3, and DG (scale bar, 50 μm). Far right panels: zoomed images showing co-localization of MAP2 with CIM6P/IGF2R (scale bar, 1 μm). (b) Rats were trained on IA (Tr) or remained in their home cages (untrained, Un) and euthanized 1 hr after training. Igf2r mRNA levels (n = 5, two independent experiments). (c) Western blot analyses comparing total and synaptoneurosomal extracts (n = 8, two independent experiments). (d) Total extracts from rats euthanized at various time points after training (30 min, 2 days, 1 week, and 2 weeks) (n = 6–8, four independent experiments). Two-tailed Student t-test or one-way ANOVA followed by Tukey’s post-hoc tests. **p<0.01 and ***p<0.001; see Source data one for detailed statistical information.

Figure 1—figure supplement 1. CIM6P/IGF2R is expressed in CaMKIIα neurons of rat hippocampus.

Immunofluorescence co-staining of CIM6P/IGF2R and CaMKIIα. Upper panels: representative composite tile scans of whole hippocampus (scale bar, 500 μm). Lower panels: CA1, CA2, CA3, and DG (scale bar, 50 μm).

Figure 1—figure supplement 2. Time course of Egr1 protein induction following IA training in rats.

Cohorts of rats were trained on IA, and euthanized 30 min, 2 days, 1 week, or 2 weeks later. Total homogenates were analyzed by western blot for Egr1 protein levels. Egr1 was induced at 30 min after training (Tr), as compared to untrained controls (Un) (n = 6–8, four independent experiments). One-way ANOVA followed by Tukey’s post hoc test. ****p<0.0001; see Source data one for detailed statistical information.