Experimental timelines are shown above graphs. Rats were injected with IgG or anti-CIM6P/IGF2R antibody before or after IA training (↑). IA acquisition (Tr) and memory retention are expressed as mean latency ± SEM (in seconds). (a) The effect of two injections of either IgG control or anti-CIM6P/IGF2R, given immediately and 8 hr after training (Tr), on IA memory tested 1 day (1 d) and 1 week after training, as well as after a reminder shock (RS; n = 6, two independent experiments). (b and c) The effect of a single injection of anti-CIM6P/IGF2R, given immediately or 8 hr after training, on memory tested 1 day and 1 week after training (n = 6–7, two independent experiments). (d and e) The effect of a single 10-fold higher dose of IgG or anti-CIM6P/IGF2R, given at the same time points, on memory tested 1 day and 1 week later (n = 6–7, two independent experiments). (f) The effect of a single injection, given 15 min before training, on memory tested 1 day and 1 week after training, as well as after a reminder shock (n = 7–8, two independent experiments). Two-way repeated measures ANOVA followed by Sidak’s post-hoc tests. ****p<0.0001; see Source data one for detailed statistical information.