Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus. musculus) | NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) | The Jackson Laboratory | Stock #: 005557, RRID:IMSR_JAX:005557 | |
| Genetic reagent (Mus. musculus) | C57BL/6J | The Jackson Laboratory | Stock #: 000664, RRID:IMSR_JAX:000664 | |
| Cell line (Homo sapiens) | PANC1 | ATCC | Cat# CRL-1469, RRID:CVCL_0480 | |
| Cell line (Homo sapiens) | PATU8988S | DSMZ | Cat# ACC-204, RRID:CVCL_1846 | |
| Cell line (Homo sapiens) | SUIT2 | JCRB | JCRB1094, RRID:CVCL_3172 | |
| Cell line (Homo sapiens) | CFPAC1 | ATCC | Cat# CRL-1918, RRID:CVCL_1119 | |
| Cell line (Homo sapiens) | AsPC1 | ATCC | CRL-1682, RRID:CVCL_0152 | |
| Cell line (Homo sapiens) | PANC0403 | ATCC | Cat# CRL-2555, RRID:CVCL_1636 | |
| Cell line (Homo sapiens) | CAPAN2 | ATCC | Cat# HTB-80, RRID:CVCL_0026 | |
| Cell line (Homo sapiens) | HPAFII | ATCC | Cat# CRL-1997, RRID:CVCL_0313 | |
| Cell line (Homo sapiens) | MIAPaca2 | ATCC | Cat# CRL-1420, RRID:CVCL_0428 | |
| Cell line (Homo sapiens) | BxPC3 | ATCC | Cat# CRL-1687, RRID:CVCL_0186 | |
| Cell line (Homo sapiens) | T3M4 | RCB | Cat# RCB1021, RRID:CVCL_4056 | |
| Cell line (Homo sapiens) | KLM1 | RCB | Cat# RCB2138, RRID:CVCL_5146 | |
| Cell line (Homo sapiens) | Human PSCs | PMID:30366930 | Primary | |
| Cell lineMus musculus | mPSC4; mPSC5 | PMID:30366930 | Primary | |
| Cell lineMus musculus | mM1 | PMID:25557080 | Primary | |
| Antibody | anti-p63 (rabbit monoclonal) | Cell Signaling | Cat# 39692, RRID:AB_2799159 | 1:1000 (WB) 1:500 (IHC) 1:500 (IF) |
| Antibody | anti-HSC70 (mouse monoclonal) | Santa Cruz Biotechnology | Cat# sc-7298, RRID:AB_627761 | 1:5000 (WB) |
| Antibody | anti-ACTB (mouse monoclonal) | Sigma-Aldrich | Cat# A3854, RRID:AB_262011 | 1:1000 (WB) |
| Antibody | anti-Cas9 (mouse monoclonal) | Epigentek | Cat# A-9000–050, RRID:AB_2828022 | 1:1000 (WB) |
| Antibody | anti-MPO (goat polyclonal) | R and D Systems | Cat# AF3667, RRID:AB_2250866 | 1:200 (IF) |
| Antibody | anti-LY6G (rat monoclonal) | BD Pharmingen | Cat# 551459, RRID:AB_394206 | 1:200 (IF) |
| Antibody | anti-MPO (rabbit polyclonal) | Abcam | Cat# ab9535, RRID:AB_307322 | 1:200 (IF) |
| Antibody | anti-alpha-SMA (mouse monoclonal) | Sigma-Aldrich | Cat# A2547, RRID:AB_476701 | 1:200 (IF) |
| Antibody | anti-Muc1 (Armenian hamster monoclonal) | Abcam | Cat# ab80952, RRID:AB_1640314 | 1:200 (IF) |
| Antibody | anti-CD45-PerCP-Cy5-5 (rat monoclonal) | BioLegend | Cat# 103132, RRID:AB_893340 | 1:100 (FC) |
| Antibody | anti-PDPN-APC/Cy7 (Syrian hamster monoclonal) | BioLegend | Cat# 127418, RRID:AB_2629804 | 1:100 (FC) |
| Antibody | anti-CD326 (EPCAM)-AlexaFluor 647 (mouse monoclonal) | BioLegend | Cat# 324212, RRID:AB_756086 | 1:100 (FC) |
| Antibody | anti-E-Cadherin-AlexaFluor 647 (rat monoclonal) | BioLegend | Cat# 147304, RRID:AB_2563040 | 1:100 (FC) |
| Antibody | anti-CD45-BV510 (rat monoclonal) | BioLegend | Cat# 103138, RRID:AB_2563061 | 1:100 (FC) |
| Antibody | anti-F4/80-BV785 (rat monoclonal) | BioLegend | Cat# 123141, RRID:AB_2563667 | 1:100 (FC) |
| Antibody | anti-Ly6G/Ly6C (Gr-1)-PE (rat monoclonal) | BioLegend | Cat# 108408, RRID:AB_313373 | 1:100 (FC) |
| Antibody | anti-CD11b-PE-Cy7 (rat monoclonal) | BioLegend | Cat# 101216, RRID:AB_312799 | 1:500 (FC) |
| Antibody | anti- CD8a-APC-Cy7 (rat monoclonal) | BioLegend | Cat# 100714, RRID:AB_312753 | 1:100 (FC) |
| Antibody | anti-CD4-APC (rat monoclonal) | BioLegend | Cat# 100516, RRID:AB_312719 | 1:100 (FC) |
| Antibody | anti-TCRβ-PE-Cy7 (Armenian hamster monoclonal) | BioLegend | Cat# 109222, RRID:AB_893625 | 1:500 (FC) |
| Antibody | Anti-CD11b-AlexaFluor 488 (rat monoclonal) | BioLegend | Cat# 101217, RRID:AB_389305 | 1:100 (FC) |
| Antibody | anti-CXCL1 (mouse monoclonal) | R and D Systems | Cat# MAB275, RRID:AB_2292460 | 10 μg/ml |
| Antibody | IgG2B Isotype Control (mouse monoclonal) | R and D Systems | Cat# MAB004, RRID:AB_357346 | 10 μg/ml |
| Recombinant DNA reagent | LentiV-Cas9-puro | Addgene | Cat# 108100 | |
| Recombinant DNA reagent | LRNG vector | Addgene | Cat# 125593 | |
| Recombinant DNA reagent | Lenti-dCas9-KRAB-blast | Addgene | Cat# 89567 | |
| Recombinant DNA reagent | YXP-Cas9-puro | This paper | See ‘CRISPR-based Targeting’ in Materials and methods | |
| Recombinant DNA reagent | Lenti-luciferase-blast vector | PMID:30428345 | ||
| Recombinant DNA reagent | LentiV-ΔNp63-neo vector | PMID:30428345 | ||
| Peptide, recombinant protein | mouse CXCL1 | Peprotech | Cat# 250–11 | |
| Peptide, recombinant protein | human CXCL1 | Peprotech | Cat# 300–11 | |
| Commercial assay or kit | IL-1α ELISA assay | R and D Systems | Cat# DLA50 | |
| Commercial assay or kit | IL-1β ELISA assay | R and D Systems | Cat# DLB50 | |
| Commercial assay or kit | IL-6 ELISA assay | R and D Systems | Cat# M6000B | |
| Other | Matrigel | Corning | Cat# 356231 | |
| Other | 3 µm FluoroBlok cell culture insert | Corning | Cat# 08-772-141 | |
| Software, algorithm | HISAT2 | PMID:25751142 | HISAT2, RRID:SCR_015530 | |
| Software, algorithm | Cuffdiff | PMID:20436464 | Cuffdiff, RRID:SCR_001647 | |
| Software, algorithm | Morpheus | Broad Institute | Morpheus by Broad Institute, RRID:SCR_017386 | |
| Software, algorithm | Metascape | PMID:30944313 | Metascape, RRID:SCR_016620 | |
| Software, algorithm | GSEA | PMID:16199517 | Gene Set Enrichment Analysis, RRID:SCR_003199 | |
| Software, algorithm | DESeq2 | PMID:25516281 | DESeq2, RRID:SCR_015687 | |
| Software, algorithm | SAMtools 1.4 | PMID:19505943 | SAMTOOLS, RRID:SCR_002105 |