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. 2020 May 5;9:e54257. doi: 10.7554/eLife.54257

Figure 4. IL-4-induced tube formation in endothelial progenitor cells (EPCs) and retinal vascular endothelial cells.

(a) IL-4-induced tube formation of retinal vascular endothelial cells. Human and murine IL-4 exposure (10 ng/ml) significantly stimulated tube formation of human and murine retinal microvascular endothelial cells in vitro, respectively. Anti-IL-4 or IL-4Rα antibodies abolished the IL-4 induced-tube formation. VEGF exposure (10 ng/ml) also stimulated tube formation. (n = 7–10/group). (b) IL-4-induced tube formation in bone marrow-derived EPCs. IL-4 exposure (10 ng/ml) significantly stimulated tube formation of EPCs. The IL-4-induced tube formation was significantly reduced in EPCs from Il4ra-/- mice but was not affected by inhibition of VEGF receptor tyrosine kinase (ZM 306416) or VEGFR-2 (ZM 323881) (n = 13–45/group). (c) Requirements of IL-4 for tube formation response of EPCs. IL-4 (10 ng/ml) and IL-13 (10 ng/ml) induced tube formation of bone marrow-derived EPCs. These actions were abolished in the EPCs from Il4-/- bone marrow cells. EPCs from Il4ra-/- mice did not respond to IL-4, however they responded to IL-13 by tube formation. EPCs from Il13ra1-/- mice did not respond to IL-13 but responded to IL-4 by tube formation. (n = 35–72/group). *p<0.05, **p<0.01, ***p<0.005, ****p<0.001, *****p<0.0005. ANOVA with post hoc test and linear mixed-effects regression analysis.

Figure 4—source data 1. IL-4-induced tube formation inEPCs and retinal vascular endothelial cells.

Figure 4.

Figure 4—figure supplement 1. IL-4Rα-mediated transcriptional networks of bone marrow-derived EPCs.

Figure 4—figure supplement 1.

(a) IL-4Rα-mediated transcriptional networks associated with angiogenesis in bone marrow-derived EPCs after IL-4 treatment. Z score = 2.781, p=1.8 × 10−4. (b) IL-4Rα-mediated transcriptional networks of bone marrow-derived EPCs. Top three highest significant networks (p<1 × 10−27) were merged and shown in hierarchical layout. Amyloid-beta A4 protein (App) and cellular tumor antigen p53 (Tp53) are shown in the top and bottom position. Red and green indicates up and down regulation, respectively. IPA was accessed on 2020/4/5.
Figure 4—figure supplement 1—source data 1. IL-4Rα-mediated transcriptional networks of bone marrow-derived EPCs.