Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) | C57BL/6J (wt) | PMID:15729571 | RRID:IMSR_JAX:000664 | |
| Genetic reagent (M. musculus) | C57BL/6-Tg(CAG-EGFP) | PMID:9175875 | RRID:IMSR_JAX:003291 | |
| Genetic reagent (M. musculus) | C57BL/6-Il4tm1Nnt/J | PMID:8906833 | RRID:IMSR_JAX:002518 | |
| Genetic reagent (M. musculus) | BALB/c-Il4ratm1Sz/J | PMID:9380721 | RRID:IMSR_JAX:003514 | |
| Genetic reagent (M. musculus) | Il13ra1tm1Twy | PMID:18066066 | RRID:MGI:3772446 | Dr. Marc E Rothenberg, Cincinnati Children's Hospital Medical Center University of Cincinnati College of Medicine |
| Cell line (M. musculus) | C57BL/6 Mouse Primary Retinal Microvascular Endothelial Cells | Cell Biologics | C57-6065 |
|
| Cell line (H. sapiens) | Primary Human Retinal Microvascular Endothelial Cells | Cell Systems | ACBRI 181 | |
| Antibody | anti IL-4 (rat monoclonal) | Biolegend | Cat. #: 504108, RRID:AB_315322 | IHC(1:200) |
| Antibody | anti IL-4 (rabbit polyclonal) | abcam | Cat. #: ab9622, RRID:AB_308736 | IHC(1:200) |
| Antibody | anti Phospho-Tyr497 IL-4R/CD124 (rabbit polyclonal) | Assay Biotechnology Company | Cat. #: A1064, RRID:AB_10683571 | IHC(1:200) |
| anti CD124 (rat monoclonal) | BD Pharmingen | Cat. #: 552288, RRID:AB_394356 | 0.1–10 ng/ mL TVI | |
| Antibody | anti IL-13 receptor alpha 1 (rabbit polyclonal) | abcam | Cat. #: ab-79277, RRID:AB_1640587 | IHC(1:200) |
| anti IL13 antibody (rabbit polyclonal) | GeneTex, Inc | Cat. #: GTX59763, | 0.1–10 ng/ mL TVI | |
| Antibody | anti CD11b (rat monoclonal) | eBioscience | Cat. #: 14-0112-82, RRID:AB_467108 | IHC(1:200) |
| Antibody | anti CD11b (rat monoclonal) Alexa Fluor 594 | Biolegend | Cat. #: 101254, RRID:AB_2563231 | IHC(1:100) |
| Antibody | CD11b (M1/70) (rat monoclonal) FITC | eBioscience | Cat. #: 11-0112-41, RRID:AB_11042156 | IHC(1:100) |
| Antibody | anti CCR2 (rabbit polyclonal) DyLight 550 | Novus Biologicals | Cat. #: NBP1-48338R | IHC(1:100) |
| Antibody | anti MCP-1 (hamster monoclonal) | Biolegend | Cat. #: 505906, RRID:AB_2071552 | IHC(1:200) |
| Antibody | anti Iba1 (rabbit polyclonal) | FUJIFILM Wako Pure Chemical Corporation | Cat. # 019–19741, RRID:AB_839504 | IHC(1:200) |
| Antibody | anti CD31 (rabbit polyclomal) | abcam | Cat. #: ab28364, RRID:AB_726362 | IHC(1:200) |
| Antibody | anti-mouse CD31(rat monoclonal) Alexa Fluor 647 | Biolegend | Cat. #: 102516, RRID:AB_2161029 | IHC(1:100) |
| Antibody | anti rat IgG (goat polyclonal) Brilliant Violet 421 | Biolegend | Cat. #: 405414, RRID:AB_10900808 | IHC(1:100) |
| Antibody | anti rabbit IgG (goat polyclonal) DyLight 488 | Vector Laboratories | Cat. #: DI-1488, RRID:AB_2336402 | IHC(1:100) |
| Antibody | anti rabbit IgG (donkey polyclonal) Alexa Fluor 555 | Biolegend | Cat. #: 406412, RRID:AB_2563181 | IHC(1:100) |
| Antibody | anti rabbit IgG (goat polyclonal) HiLyte Fluor 555 | AnaSpec | Cat. #: AS-61056–05 H555 | IHC(1:100) |
| Antibody | anti hamster IgG (goat polyclonal) DyLight 594 | Biolegend | Cat. #: 405504, RRID:AB_1575119 | |
| Antibody | anti rabbit IgG (goat polyclonal) PE | Santa Cruz Biotechnology | Cat. #: sc-3739, RRID:AB_649004 | IHC(1:100) |
| Antibody | anti rabbit IgG (donkey polyclonal) Alexa Fluor 647 | abcam | Cat. #: ab150075, RRID:AB_2752244 | IHC(1:100) |
| Antibody | anti rabbit IgG (donkey polyclonal) DyLight 649 | Biolegend | Cat. #: 406406, RRID:AB_1575135 | IHC(1:100) |
| Antibody | anti mouse IgG2A (rat monoclonal) | R and D | Cat. #: mab006, RRID:AB_357349 | 0.1–10 ng/ mL TVI |
| Antibody | anti mouse Fc gamma RII/RIII (CD32/CD16)(goat polyclonal) | R and D | Cat. #: AF1460-SP, Accession # P08101 | IHC (0.2 μg/mL) |
| Peptide, recombinant protein | recombinant murine IL-4 | R and D | Cat. #: 404 ML | |
| Peptide, recombinant protein | recombinant human IL-4 | Peprotec | Cat. #: AF-200–04 | |
| Peptide, recombinant protein | recombinant murine IL-13 | Peprotec | Cat. #: 210–13 | |
| Chemical compound, drug | ZM306416 hydrochloride | abcam | Cat. #: ab144576 | |
| Chemical compound, drug | ZM323881 hydrochloride | R and D | Cat. #: 2475/1 | |
| Chemical compound, drug | bovine serum albumin | Sigma-Aldrich | Cat. #: A2153 | |
| Chemical compound, drug | fetal bovine serum | Sigma-Aldrich | Cat. #: 12103C | |
| Chemical compound, drug | Medetomidine | Chemscene LLC | Cat. #: 86347-14-0 | |
| Chemical compound, drug | Butorphanol tartrate | FUJIFILM Wako Pure Chemical Corporation | Cat. #: 58786-99-5 | |
| Chemical compound, drug | Midazolam | FUJIFILM Wako Pure Chemical Corporation | Cat. #: 59467-70-8 | |
| Chemical compound, drug | Tropicamide, Phenylephrine Hydrochloride | Santen Pharmaceuitical Co., Ltd. | Cat. #: 1319810Q1053 | |
| Chemical compound, drug | Hydroxy methyl cellulose | SENJU Pharmaceutical Co.,Ltd | Cat.#: 131980AQ1038 | |
| Chemical compound, drug | RNAlater solution | Ambion | Cat. #: AM7021 | |
| Chemical compound, drug | Triton X-100 | Sigma-Aldrich | Cat. #: X100 100ml | |
| Chemical compound, drug | Tween 20 | Sigma-Aldrich | Cat. #: P1379-100ml | |
| Chemical compound, drug | Paraformadehyde | Electron Microscopy Science | Cat. #: 15710 | |
| Commercial assay or kit | ISOLECTIN B4 Fluorescein | Vector Laboratories | Cat. #: FL-1201, RRID:AB_2314663 | IHC(1:100) |
| Commercial assay or kit | ISOLECTIN B4 DyLight 594 | Vector Laboratories | Cat. #: FL-1207 | IHC(1:100) |
| Commercial assay or kit | DAPI | Roche Diagnostics | Cat. #: 10 236 276 001 | 1 μg/ml |
| Commercial assay or kit | TO-PRO-3 iodide | Molecular Probes, Inc | Cat. #: T-3605 | IHC(1:100) |
| Commercial assay or kit | VECTASHIELDAntifade Mounting Medium | Vector Laboratories |
Cat. #: H-1000, RRID:AB_2336789 | |
| Commercial assay or kit | Fluorescence Mounting Medium | DAKO | Cat. #: 15710 | |
| Commercial assay or kit | SurePrint G3 Mouse GE 8 × 60K Microarray | Agilent Technologies | Cat. #: AGLMO002 | |
| Commercial assay or kit | QuantiTect Reverse Transcription Kit | Qiagen | Cat. #: 205311 | |
| Commercial assay or kit | QuantiTect SYBR Green PCR kit | Qiagen | Cat. #: 204143 | |
| Commercial assay or kit | ELISA kits | ThermoFisher Scientific | Cat. #: BMS6005, EMFLT1 | |
| Commercial assay or kit | PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling | Sigma-Aldrich | Cat. #: PKH26GL-1KT | |
| Commercial assay or kit | RNeasy Mini Kit | Qiagen | Cat. #: 74104 | |
| Commercial assay or kit | DMEM:F12 | ThermoFisher Scientific | Cat. #: 11330057 | |
| Commercial assay or kit | recombinant human GMCSF | Peprotec | Cat. #: AF-300–03 | |
| Commercial assay or kit | recombinant murine GMCSF | Peprotec | Cat. #: 315–03 | |
| Software, algorithm | Ingenuity Pathway Analysis, March 2020 | Qiagen | RRID:SCR_008653 | |
| Software, algorithm | Adobe Photoshop CS5, Version 12.0.5 | Adobe | RRID:SCR_014199 | |
| Software, algorithm | STATA 16.1 | StataCorp LLC | RRID:SCR_012763 | |
| Software, algorithm | GeneSpring Software | Agilent Technologies | RRID:SCR_009196 |