Skip to main content
. 2020 May 5;9:e54160. doi: 10.7554/eLife.54160

Figure 5. Nafcillin Sodium affects ergosterol levels.

Figure 5.

Molecular structures of beta-lactam antibiotics shown for (A) Nafcillin Sodium (B) Cefazolin (C) Cefonicid (D) Cefoxitin (E) Methicillin (F) Aztreonam (G) Oxacillin (H) Carbenicillin (I) Azlocillin (J) Ampicillin (K) Amoxicillin (L) Amdinocillin. (M) FICI scores for 50% inhibition of C. neoformans of various antibiotics related to nafcillin sodium tested with fluconazole. Violet bars over the red line illustrate a FICI score of ≥4 indicating antagonism. (N) Ergosterol biosynthesis pathway illustrating cytochrome P450 enzymes. (O) Ergosterol quantification from cell treated with Nafcillin (NAF), FLZ, or NAF+FLZ. Data normalized to control treated. *=p value is 0.03 (Mann-Whitney test). All average FICI scores represent an average of at least two independent tests (technical and biological replicates). Source data are in Figure 5—source data 1.

Figure 5—source data 1. FICI scores of small molecules with structures similar to nafcillin.
FICI scores for small molecules listed in combination with fluconazole. All scores are for FICI 90% inhibition unless listed. *=FICI scores for 50% inhibition (when 90% inhibition of fungal growth could not be obtained.
Figure 5—source data 2. FICI scores of nafcillin in combination with amphotericin B.
FICI scores for 50% inhibition for nafcillin in combination with amphotericin B for KN99 C. neoformans.