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. 2020 Apr 16;9:e55015. doi: 10.7554/eLife.55015

Figure 2. Cryo-EM structure of Dvl2 DIX filaments reveals anti-parallel double helices stabilized by intra- strand (head-tail) and inter-strand contacts.

(A) Cryo-EM image of purified Dvl2 DIX filaments. (B) Final sharpened cryo-EM map with helical symmetry imposed, with the two antiparallel strands shown in green and blue contours. (C) Final model of the Dvl2 DIX anti-parallel double helix, containing 12 subunits. The secondary structure elements are marked in the upper brown copy. The schematic diagram on the right shows the antiparallel structure, with each subunit represented as a triangle colored as in (B). (D) A portion of the final cryo-EM map (wire frame) and model showing a head-to-tail interface involving Y27 (head) packing against F56 and K68 (tail).

Figure 2.

Figure 2—figure supplement 1. Cryo-EM analysis of Dvl2 DIX filaments.

Figure 2—figure supplement 1.

(A) Representative 2D class averages of sections of filaments in a 150 Å by 150 Å box. (B) 3D classes. Particles belonging to the boxed 3D classes were used for the final reconstruction. (C) Unsharpened and sharpened maps. (D) Fourier Shell correlation coefficient as a function of resolution. The resolution is indicated at FSC = 0.143. (E) Local resolution estimation displayed on the sharpened map. (F) A representative subunit in map density, contoured at 2.7σ. (G) Map-to-model Fourier Shell correlation coefficients as a function of resolution. The resolution is indicated at FSC = 0.5.