Table 3.

Recoveries of 1-NAP from spiked urine samples by icELISA and HPLC-FLD (n = 3)^a

Spiked (ng/mL)	ciELISA			HPLC-FLD
	mean(ng/mL) (mean±SDb)	Recovery (%)	CVc (%)	mean(ng/mL) (mean±SD)	Recovery (%)	CV (%)
0d	1.1±0.2	NDe	18.1	0.7±0.1	ND	14.3
600	741.3±86.9	123.4	11.7	744.7±30.2	123.9	4.1
200	207.0±30.5	102.9	14.7	184.2±9.0	91.5	4.9
60	62.6±9.0	102.4	14.4	55.6±1.2	90.8	2.2

^aFor one concentration, three positive samples were spiked and determined by ICA and LC

^bSD, standard deviation

^cCV, coefficient of variance, which was obtained from intra-assay.

^dBlank control

^eND, not detected