Subject	Nanotechnology
Specific subject area	Super-resolution microscopy
Type of data	Time-lapse records
	Lists of single-molecule localization space-time coordinates
	Matlab functions
How data were acquired	Total internal reflection fluorescence microscope
	Instrument: Nikon N-STORM microscope.
	Softwares: NIS-elements (Nikon)
Data format	Raw
	analyzed
Parameters for data collection	Particles on glass coverslip were imaged with continuous laser excitation (647 nm) under total internal reflection conditions. Fluorescence time-lapse records of 10,000–50,000 frames were acquired at 10–20 Hz onto a region of the camera of 40 µm in size.
Description of data collection	Binding sites exposed on streptavidin-coated polystyrene particles were loaded with biotin-conjugated short single strands of DNA and seeded on glass coverslip. A complementary free single strand of DNA, conjugated with a fluorophore, was introduced at low concentration (0.1 – 5 nM) in solution and time-lapse fluorescence imaging acquisitions were recorded.
Data source location	Institution: Institute for Bioengineering of Catalonia (IBEC)
	City: Barcelona
	Country: Spain
Data accessibility	In a public repository
	Repository name: 4TU-center for Research Data
	Data identification number: uuid:50bd0ad4-52d8-4138-8eb1-40b4d7fc2286
	Direct URL to data: https://doi.org/10.4121/uuid:50bd0ad4-52d8-4138-8eb1-40b4d7fc2286
Related research article	Delcanale et al., Nanoscale Mapping Functional Sites on Nanoparticles by Points Accumulation for Imaging in Nanoscale Topography (PAINT), ACS Nano, 10.1021/acsnano.7b09063 [1]