Figure 5.

Human monocytes are recruited to organoids and acquire an intermediate‐like phenotype. Human monocytes were mixed with mature organoids and cocultured in Matrigel for 72 h. Prior to coculture with monocytes, organoids were devoid of CD45⁺ hematopoietic cells, as assessed by flow cytometry, while after coculture, a population of CD45⁺ CD13⁺ cells was associated with both organoid types (a). Gated on singlets, live cells. Confocal analysis confirms that cocultured CD45⁺ monocytes interact closely with lung (b) and intestinal organoids (c), showing an elongated morphology typical of tissue‐infiltrating monocytes (maximum intensity projection, scale bar 100 μm). Monocytes cocultured with organoids showed a more intermediate‐like surface marker phenotype than monocytes cultured on plastic or alone in Matrigel, as indicated by the expansion of the CD14⁺ CD16⁺ fraction, and the higher expression of CD14 and HLA‐DR (d–f). Gated on FSC/SSC, singlets, live cells, CD45⁺. Results of three independent experiments are shown.