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. 2020 May 1;15:3087–3098. doi: 10.2147/IJN.S241324

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© 2020 Zhou et al.

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CS-4D5/6e targets AKR1C3, and overexpression of AKR1C3 enhances the cytotoxic effect of CS-4D5/6e on cells. Percentage cell viability of (A) LNCAP cells cultured in normal and CSS media, (B) LNCaP-AKR1C3 cells cultured in normal, CSS, and CSS media supplemented with 10 nM Δ4-androstenedione after CS-4D5/6e treatment for 24 h. Percentage cell viability of (C) LNCAP cells cultured in normal and CSS media, (D) LNCaP-AKR1C3 cells cultured in normal, CSS, and CSS media supplemented with 10 nM Δ4-androstenedione after 6e treatment for 24 h. (E) Western blot analysis the expression of AKR1C3, PSA, and AR after LNCAP-AKR1C3 cells treated with CS-4D5/6e. Treatment with CS-4D5/6e at indicated concentration for 24 h in LNCaP-AKR1C3 cells cultured in CSS media.

Abbreviations: CS, chemically chitosan; 4D5, single chain antibody fragment 4D5; 6e, a derivative of mansonone F; AD, androgen; CSS, charcoal-stripped serum; PSA, prostate specific antigen; AKR1C3, aldo-ketoreductase1C3; AR, androgen receptor; Ctr, control.