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. 2020 May 1;13:3677–3687. doi: 10.2147/OTT.S242462

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© 2020 Li et al.

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SCAMP3 promotes glioma cell proliferation. (A) The proliferation curves of transfected U251 cells were plotted based on MTS data, which indicated that overexpressing SCAMP3 enhanced while silencing SCAMP3 inhibited cell proliferation. (B) Colony formation assays were conducted for transfected U251 cells. Consistent with the proliferation results, SCAMP3 transfection resulted in more colonies while SCAMP3-knockdown attenuated colony formation. (C) Immunoblotting results confirmed the transfection efficiencies of SCAMP3 overexpression and knockdown. Besides, silencing SCAMP3 leads to impaired EGFR recycling, as well as the activation of S6K and 4E-BP1 proteins. (D) U251 cells transfected with SCAMP3 plasmids were further treated with rapamycin or gefitinib and then subjected to MTS assay. Either inhibiting mTOR or EGFR can significantly attenuate the proliferation-promoting effect of SCAMP3 on glioma cells. (E) The alteration of migration capacity by overexpressing or silencing SCAMP3 was tested and revealed no statistical significance. (F) Matrigel-transwell assay demonstrated that SCAMP3 has no significant effect on U251 cell invasion. Data were analyzed by Student’s t-test compared with control group. *P<0.05, **P<0.01. NS, not significant.