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Expression and secretion of substance P is significantly increased in TG neurons after desiccating stress. Expression of CGRP (A), alpha-MSH(B) and substance P (C) mRNA in TG tissue from naïve and DED mice, n=5. (D) SP protein expression in TG tissue was measure with ELISA, n=5. (E) SP mRNA expression in cultured TG neurons isolated from naïve and DED mice, n=8. (F) Protein level of SP secreted by cultured TG neurons in the conditioned media, n=5. *p<0.05, **p<0.01.
