Figure 6:

En face confirmation of intact endothelium after treatment with 5 U/mL of Neur. A and B. Expression of eNOS before and after the treatment with Neur enzyme. Images show that eNOS is expressed around the nucleus of ECs. C and D. E-selectin coverage before and after the treatment with enzyme. E-selectin is expression across the surface of the endothelium. E and F. PECAM-1 staining before and after the treatment of enzyme. Visual inspection show the presence of PECAM-1 across the entire surface of the endothelium. G and H. ZO-1 staining before and after enzyme treatment. Images show the endothelial cell-cell boundaries clearly and confirm endothelial barrier integrity. I. Data quantification for E-selectin showing a non-significant difference in expression of E-selectin on the endothelium between control and enzyme treated mice. All data are normalized with UF results. Student’s t test was used to compare endothelium from untreated mice to endothelium from Neur-treated mice. The sample size (N) is 4. NS denotes “not significant”.