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. 2020 Apr 21;34:101542. doi: 10.1016/j.redox.2020.101542

Fig. 5.

Fig. 5

MnTE-2-PyP enhanced NRF2 levels. Human prostate fibroblast cells were treated with 20 mM glucose (HG) in the presence or absence of 3 Gy of X-rays (RAD) and 30 μM MnTE-2-PyP (T2E). A. Total NRF2 protein expression. T2E increased total NRF2 protein levels in whole cell extracts in irradiated and non-irradiated conditions. B. NRF2 protein visualized by immunofluorescence. Immunofluorescence staining showed a decrease in NRF2 (green fluorescence) in irradiated and HG treated cells and T2E enhanced NRF2 expression. In irradiated cells, T2E treatment enhanced nuclear (blue) localization of NRF2. The scale bar = 100 μm. C. Cytosolic NRF2 protein expression. Cytosolic NRF2 levels were significantly lower in the RAD + HG group as compared to the HG group and T2E treatment increased cytosolic NRF2 levels. D. Nuclear NRF2 protein expression. Nuclear NRF2 levels were significantly lower in radiated conditions as compared to control. T2E treatment significantly upregulated nuclear NRF2 levels in control, HG, RAD and RAD+HG conditions. n ≥ 3. (*) denotes a significant difference as compared to control, ($) denotes a significant difference as compared to HG, (#) denotes a significant difference as compared to RAD and (@) denotes a significant difference as compared to RAD + HG group. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)