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. 2018 Mar 19;2(2):195–204. doi: 10.1093/tas/txy008

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© The Author(s) 2018. Published by Oxford University Press on behalf of the American Society of Animal Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Regression analyses for total motility in Exp1. Semen was collected from six boars and diluted into four treatment extenders that were supplemented with 0, 51, 102, and 205 mM of betaine and analyzed on day of collection (D0) and after 72 h of storage (D3). The mixed procedure of SAS 9.4 was used to perform the regression analyses, where three regressions were performed: overall total motility, D0 total motility, and D3 total motility. All regressions were quadratic. For the overall total motility regression, the following equation was determined to best fit the data: y = 71.3 + 0.19x – 0.0013x², where x = mM betaine; R² = 0.52; maximum total motility = 77.8% when x = 70 mM. For fresh semen (D0), the following equation best fits the data: y = 72.2 + 0.19x – 0.0011x², where x = mM betaine; R² = 0.55; maximum total motility = 80.8 when x = 90 mM. For stored semen (D3), the following equation best fits the data: y = 70.4 + 0.18x – 0.0016x², where x = mM betaine; R² = 0.71; maximum total motility = 75.4 when x = 55 mM.