Table 2.

Refractive index and pH LS means for Exp2¹

	Treatment extender2						SE	P values
	ST0	ST70	BSA0	BSA70	LT0	LT70		BET	EXT	BET × EXT
Refractive index, brix score	4.6	5.3	4.6	5.5	4.1	4.9	0.04	<0.0013	<0.001	0.661
pH	7.1a	7.1b	6.7c	6.7c	6.9d	6.9d	0.01	0.052	<0.001	0.024

BET = betaine concentration; EXT = extender type.

¹ Columns within rows separated by differing letters are different, P < 0.05.

² Different commercial semen extenders with supplemental betaine; short-term 0 mM, ST0; short-term 70 mM, ST82; long-term containing bovine serum albumin 0 mM, BSA0; long-term containing bovine serum albumin 70 mM, BSA70; long-term not containing bovine serum albumin 0 mM, LT0; long-term not containing bovine serum albumin 70 mM, LT70.

³ Refractive index was greater for 70 mM compared with 0 mM of betaine (5.4 vs. 4.4 ± 0.05).