Table 4.

Computer-assisted sperm assessment LS means for Exp2¹

	Treatment extender						SE	P Values
	ST0	ST70	BSA0	BSA70	LT0	LT70		BET	BET × EXT	BET × Day
Mobility parameters
ALH, µm	4.0	4.5	5.2	5.4	5.0	5.4	0.7	0.009	0.639	0.325
BCF, µm/s	35.7	33.3	35.9	34.3	35.7	33.5	1.4	<0.001	0.451	0.004
DAP, µm	25.3	26.8	28.5	28.9	30.0	30.7	3.1	0.239	0.778	0.125
DCL, µm	39.1	42.8	47.8	48.3	48.7	50.1	5.8	0.152	0.624	0.264
DSL, µm	21.0	21.9	24.4	24.2	25.0	25.3	2.3	0.528	0.776	0.169
LIN, %	57.2	55.1	51.4	51.2	53.6	52.8	3.3	0.217	0.639	0.803
STR, %	83.7	81.9	84.9	83.7	83.5	82.6	1.8	0.009	0.738	0.275
VAP, µm/s	64.3	69.3	66.7	68.2	74.4	76.4	8.3	0.150	0.744	0.081
VCL, µm/s	99.7	110.4	110.9	113.5	120.2	124.3	15.8	0.115	0.635	0.185
VSL, µm/s	53.9	57.1	57.1	57.4	62.3	63.6	6.3	0.302	0.750	0.088
WOB, %	66.2	64.8	60.3	60.8	63.2	62.7	2.6	0.564	0.585	0.822
Motility parameters
Total motility, %	75.9	70.9	84.2	81.8	86.7	84.0	4.5	0.010	0.668	0.174
Progressive motility, %	69.6	64.1	79.9	76.8	80.2	77.3	3.8	0.001	0.567	0.246

ST0 = short-term 0 mM; ST70 = short-term 70 mM; BSA0 = long term with bovine serum albumin 0 mM; BSA70 = long term with bovine serum albumin 70 mM; LT0 = long-term 0 mM; LT70 = long-term 70 mM; BET = betaine concentration in extender; EXT = extender type; Day = day of semen analysis; ALH = anterior lateral head displacement; BCF = beat cross frequency; DSL = straight-line distance; DAP = average path distance; DCL = curvilinear distance; LIN = linearity; STR = straightness; VSL = straight-line velocity; VAP = average path velocity; VCL = curvilinear velocity; WOB = wobble.

¹ Columns within rows separated by differing letters are different (P < 0.05) for BET × EXT effect.