Table 3.

Milk production from lactating ¾ Holstein × ¼ Gir dairy cows assigned to receive (SUPP; n = 18) or not (CON; n = 18) 30 g/cow daily (as-fed basis) of a supplement containing condensed tannins, essential oils, and pungent compounds (Actifor Pro; Delacon Biotechnik GmbH; Steyregg, Austria) from d 0 to 84 of the experiment^a

Item	SUPP	CON	SEM	P-value
Milk production, kg/d
Milk yield	29.5	27.9	0.5	0.04
Fat-corrected milk	32.6	30.0	0.7	0.02
Energy-corrected milk	33.0	30.5	0.7	0.01
Milk composition
Fat, %	4.10	4.01	0.10	0.54
Protein, %	3.34	3.35	0.03	0.69
Casein, %	2.58	2.59	0.02	0.74
Milk urea N, %	13.3	13.5	0.3	0.67
Lactose, %	4.66	4.59	0.04	0.15
Total solids, %	13.0	12.9	0.17	0.80
Somatic cell count, cells/μLb	5.02	5.11	0.08	0.45
Milk nutrient output
Fat, kg/d	1.22	1.11	0.03	0.04
Protein, kg/d	0.979	0.927	0.021	0.10
Casein, kg/d	0.757	0.716	0.016	0.09
Lactose, kg/d	1.38	1.28	0.03	0.04
Total solids, kg/d	3.84	3.63	0.10	0.15
Energy, Mcal/d	22.3	20.5	0.5	0.01

^aIndividual milk production was recorded daily from d –7 to 84. Milk samples were collected once weekly from each cow following each milking of the day as in Rodrigues et al. (2018). Fat-corrected milk (FCM) and energy-corrected milk (ECM) were calculated according to the NRC (2001), based on milk concentrations of fat, protein, and total solids of the concurrent week. Milk nutrient output was estimated based on milk composition, daily milk yield, and energy output (NRC, 2001). Values obtained from d –7 and 0 were averaged and included as covariate within each respective analysis; therefore, values reported are covariately adjusted least square means.

^bOriginal somatic cell count results were not normally distributed (W = 0.55); therefore, were log transformed (base-10 log) to achieve normality (W = 0.99).