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. 2019 Feb 26;3(2):842–854. doi: 10.1093/tas/txz023

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© The Author(s) 2019. Published by Oxford University Press on behalf of the American Society of Animal Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — The effects of heat stress (HS) and zinc exposure on claudin-4 expression in IPEC-J2 cells treated with different treatments and exposed to HS at 41.5 °C for 48 h. (A) Thermoneutral (TN) condition. (B) HS condition. (C) HS and treated with zinc sulfate. (D) HS and treated with ZnB. (E) HS and treated with CaB. (F) Semiquantification of claudin-4 protein abundance and nuclear stain DAPI in the IPEC-J2 cells exposed to different treatments and HS. Red color indicates claudin-4; blue color indicates nuclei. Values are expressed as mean ± standard error of the mean.