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. 2020 Apr 21;17:306–319. doi: 10.1016/j.omto.2020.04.007

Figure 2.

Figure 2

DNMT3B Is Upregulated after Exposure to Ionizing Radiation and Involved in Radioresistance of NPC

(A) Representative images from colony formation assay, showing survival fraction of CNE2-R cells and CNE2 cells with radiation. (B) DNMT3B expression of CNE2-R and CNE2 cells detected by immunoblotting. (C) The proteins of DNMT family members from whole-cell lysates were analyzed using immunoblotting in different NPC cell lines after irradiation. (D) Quantitative analysis of mRNA of DNMT family members in different NPC cell lines after irradiation. (E) DNMT3B protein levels were analyzed by immunoblotting in different NPC cell lines. (F and G) Selection of the most appropriate NPC cell lines and verification of the silencing efficiency of shDNMT3Bs through immunoblotting (F) and RT-PCR (G). (H) Expression levels of DNMT1, DNMT3A, and DNMT3B after silencing DNMT3B in CNE2 and HNE1 cell lines. (I) Representative images from colony formation assay showing cell viability after silencing DNMT3B. ∗p < 0.05, ∗∗p < 0.01.