Figure 3. PolyP is a substitute for ATP in a F0F1-ATPase assay.

(A) Short-chain polyP did not increase the ATPase activity, whereas MpolyP (B) and LpolyP (C) at both 5 and 10 mM concentrations, significantly decreased the absorbance at 340 nm overtime, corresponding to an increased consumption of polyP by the F0F1-ATPase (raw traces). 2 µg/ml oligomycin inhibit the effects of MpolyP (B) or LpolyP (C) on the F₀F₁-ATPase activity. (D) Quantification diagram of the rate of ATPase activity, shown as OD/min of the experiments, partially represented on (A C). ***, P < 0.0001.