Fig. 8. Genetic control of the msh1 effect.

Methylation/demethylation and silencing pathway component methylation (a) and expression changes (b) in msh1 memory. Genes associated with differentially expressed sRNA clusters were highlighted with a green star. c Double mutants of msh1 with components of the RdDM pathway. d Embryo lethality in the msh1/hda6 double mutant. Methylation/demethylation and silencing pathway component gene list was taken from Matzke et al.⁵. d Representative plants of WT, drm1/2, drm1/2 MSH1 RNAi (+), drm1/2 MSH1 RNAi transgene null segregant and msh1 memory (12 h day length, 6-week-old plants). e Chlorophyll measurements, and f days to bolting in wild type (WT), msh1 memory, drm1/2 and drm1/2 MSH1 RNAi transgene null segregant. Bars represent means ± SE, n represents number of plants in each population. One-sided Mann−Whitney test was used to compute p value. Significance codes: ***(p value < 0.001), ns not significant. Plants grown on normal soil conditions. Chlorophyll level was measured with a SPAD-502 meter 4 weeks after planting. Source data underlying Fig. 8e, f are provided as a Source Data file.