Table 1.
Absolute quantification of tRF levels in plasma samples from epilepsy patients.
| 5′AlaTGC | Patient | Molarity | 5′GluCTC | Patient | Molarity | 5′GlyGCC | Patient | Molarity |
|---|---|---|---|---|---|---|---|---|
| Pre-seizure | E1 | 0.44aM | Pre-seizure | E1 | 0.01 zM | Pre-seizure | E1 | 0.13 zM |
| E2 | 2.65 aM | E2 | 0.9 zM | E2 | 0.17 zM | |||
| E3 | 324.02 pM | E3 | 0.06 zM | E3 | 19.81 pM | |||
| E4 | 0.06 aM | E4 | 0.01 zM | E4 | 0.23 zM | |||
| E5 | 3.67 aM | E5 | 0.01 zM | E5 | 13.07 pM | |||
| E6 | 0.41 aM | E6 | 11.75 fM | E6 | 36.66 pM | |||
| Post-seizure | E1 | 0.05 aM | Post-seizure | E1 | 0.01 zM | Post-seizure | E1 | 0.80 zM |
| E2 | 9.22 zM | E2 | 0.01 zM | E2 | 1.08 zM | |||
| E3 | 328.27 aM | E3 | 0.01 zM | E3 | 2.49 fM | |||
| E4 | 0.02 aM | E4 | 0.01 zM | E4 | 0.34 aM | |||
| E5 | 86.85 aM | E5 | 27.82 aM | E5 | 2.83 zM | |||
| E6 | 0.99 aM | E6 | 0.01 zM | E6 | 1.03 zM |
Changes in current were used to calculate absolute levels of tRFs using the curves shown in Fig. 3. The majority of pre-seizure samples showed levels above the LOD however some post seizure samples were below the estimated LODs.