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. 2020 May 5;10:7516. doi: 10.1038/s41598-020-64485-4

Table 2.

Oligonucleotides for tRNA fragment detection. Mismatched bases are underlined and in bold.

5AlaTGC:
Target: 5′–GGGGATGTAGCTCAGTGGTAGAGC-3′
Capture: SH - C6–5′-GCTCTACCACTGAGCTACA-3′
Probe: 5′–TCCCC - 3′ - C6 - SH
Mismatch: 5′-GGG GAA TTA GCT CAA ATG GTA GA–3′
5GlyGCC:
Target: 5′–GCATGGGTGGTTCAGTGGTAGAATT-3′
Capture: 5′-TACCACTGAACCACCCATGC-3′ - C6 - SH
Probe: SH - C6–5′–AATTC-3′
Mismatch 1: 5′-GCG TTG GTG GTA TAG TGG TGA GCA T–3′
Mismatch 2: 5′-GCA TTG GTG GTT CAA TGG TAG AAT T–3′
5GluCTC:
Target: 5′–TCCCTGGTGGTCTAGTGGTTAGGATT-3'
Capture: 5′–TAACCACTAGACCACCAGGGA-3′- C6 - SH
Probe: SH - C6–5′-AATCC-3′
Mismatch: 5′-TCC CAC ATG GTC TAG CGG TTA GGA TT–3′