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. 2020 May 5;10:7563. doi: 10.1038/s41598-020-64298-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Twist1 mediates PDGFB expression in IPAH patient ECs in vitro. (a) Graph showing the mRNA levels of Twist1 and PDGFB in PAECs derived from IPAH patients or healthy controls (left; n = 4–6, *p < 0.05). Graph showing the protein levels of PDGFB in PAECs derived from IPAH patients or healthy controls (right; n = 4–6, *p < 0.05). Error bars represent s.e.m. (b) Graph showing the mRNA levels of Twist1 and PDGFB in PAECs derived from IPAH patients or healthy controls or in combination with treatment with control siRNA or Twist1 siRNA (left; n = 4–6, *p < 0.05). Graph showing the protein levels of PDGFB in PAECs derived from IPAH patients or healthy controls or in combination with treatment with control siRNA or Twist1 siRNA (right; n = 4–6, *p < 0.05). Error bars represent s.e.m.