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. 2020 May 5;10:7540. doi: 10.1038/s41598-020-64486-3

Table 1.

Tests for the stability of miR-375 stored in LDCVs.

Treatment Reactions Control +RNase A +TX-100 + RNase A +TX-100 + Proteinase K + RNase A
Vol. Final Conc. Vol. Final Conc. Vol. Final Conc. Vol. Final Conc.
LDCVs 5 µl 50 µg 5 µl 50 µg 5 µl 50 µg 5 µl 50 µg
Syn-cel-miR-39 5 µl 0.5 pmol 5 µl 0.5 pmol 5 µl 0.5 pmol 5 µl 0.5 pmol
RNase-DNase Free PBS 10 µl 7.5 µl 5.5 µl 6 µl
10% TX-100 (vol/vol) 2 µl 1% 2 µl 1%
Proteinase K(1 mg/ml) 2 µl 100 µg/ml
Protease inhibitor (2×) 10 µl
RNase-DNase Free PBS 5 µl
RNase A (100 µg/ml) 2 µl 10 µg/ml 2 µl 10 µg/ml 4 µl 10 µg/ml
RNase inhibitor (40 U/µl) 0.5 µl 1 U/µl 0.5 µl 1 U/µl 1 µl 1 U/µl
Final Volume 20 µl 20 µl 20 µl 40 µl