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. 2020 May 5;10:7504. doi: 10.1038/s41598-020-63305-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fst values between the subspecies in different locations plotted along chromosomes. The patterns of genetic differentiation across the genome are more or less uniform. Overall, Fst values are higher between the subspecies than between autogenic and anautogenic Cx. p. pipiens from Washington, D.C., USA. P and q stand for short and long chromosome arms, respectively.