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. 2020 May 5;10:7605. doi: 10.1038/s41598-020-64621-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CM nucleation and nuclear ploidy. (A) Evaluation of CM mononuclear percentage of male and female mice of the BALB/cJ (abbreviated as cJ) and BALB/cByJ (abbreviated as cByJ) parental strains. 3 data points for BALB/cJ females and 3 data points for BALB/cByJ females are from a prior analysis⁹, all others were newly generated in this analysis. (B) Evaluation of the nuclear ploidy specifically of the mononuclear CM subpopulation of the parental strains. The data points graphed are measurements of the percentages of diploid nuclei in individual mice. (C) Evaluation of hepatocyte mononuclear percentage in the two substrains. (D) Evaluation of the nuclear ploidy specifically of the mononuclear hepatocyte subpopulation. (E) Evaluation of CM mononuclear percentage of male and female F1 mice bred from crosses of the two parental strains. (F) Evaluation of nuclear ploidy of the mononuclear CM subpopulation of F1 mice. Primary data for panels B and F are in Supplementary Fig. S1 and S4. Error bars in all panels are standard deviation.