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. 2020 May 5;10:7566. doi: 10.1038/s41598-020-64508-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NAT2 mRNA and protein expression in the cryopreserved human hepatocytes. Relative NAT2 mRNA and protein expression were quantified as described in the methods section. Each dot represents an individual sample. The line represents the median relative expression in the corresponding group. (a) Relative NAT2 mRNA expression in the rapid, intermediate and slow acetylation genotypes. (b) Relative NAT2 mRNA expression in specific rapid (NAT2*4/*4), intermediate (NAT2*4/*5; NAT2*4/*6) and slow (NAT2*5/*5; NAT2*6/*6) acetylator genotypes. (c) Relative NAT2 protein expression using monoclonal antibody ab194114, in the cryopreserved human hepatocytes of rapid, intermediate and slow acetylator NAT2 genotypes. (d) Relative NAT2 protein expression using monoclonal antibody ab194114, in rapid (NAT2*4/*4), intermediate (NAT2*4/*5; NAT2*4/*6) and slow (NAT2*5/*5; NAT2*6/*6) acetylator genotypes. *p < 0.05, **p < 0.01, ***p < 0.001.