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. 2020 May 5;10:7543. doi: 10.1038/s41598-020-63672-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) RT-PCR analysis of the AR transcript. NTC: Negative control; C+: Positive control; B: Non-spiked blood sample; M: Molecular weight marker; 50: Blood sample spiked with 50 LNCaP cells; 250: Blood sample spiked with 250 LNCaP cells; 500: Blood sample spiked with 500 LNCaP cells; 1000: Blood sample spiked with 1000 LNCaP cells. (b) ACTB was used as a control to assess cDNA synthesis. Images of the DNA stained gels were acquired using an exposure time of 330 ms and cropped to provide clarity, uncropped images were included in Supplementary Fig. S3. The AR point mutation T878A was identified by comparing the electropherogram obtained after processing a blood sample spiked with 50 LNCaP cells (c) with the one acquired from a suspension containing only PC-3 cells (d). The underline denotes the nucleotides comprising the AR 878 codon and the asterisks indicate the nucleotide that is switched.