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. 2020 May 5;10:7523. doi: 10.1038/s41598-020-64574-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Principle component analysis of the fecal microbiota composition of 85 overweight insulin resistant overweight males from Maastricht (MAA) and Amsterdam (AMS). Individuals from AMS and a subset from MAA overlap and a second group of individuals in MAA was observed as indicated by the right ellipse. These also show associations with the two metabolic parameters associated with microbiota composition in MAA through Random Forests analysis. The direction of the species arrows depicts the abundance of microbial groups. Length of the arrows is a measure of fit. The environmental variable arrows approximate the correlation between species and an environmental variable. The further a sample falls in the direction indicated by the arrow, the higher the correlation. Samples near the coordinate origin (zero point) suggest near zero correlation.