Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 5;10:7604. doi: 10.1038/s41598-020-64348-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

RU.521 acts upstream of cGAMP and downstream of HT-DNA in the cGAS-STING signaling axis. THP-1 KO cGAS cells were treated with HT-DNA or cGAMP in the absence and presence of RU.521 (0.8 µM) (a, left panel). In a parallel assay, WT THP-1 cells were exposed to HT-DNA +/− RU.521 followed by RT-qPCR analysis for IFNB1 expression (a, right panel). WT THP-1 Lucia ISG cells were stimulated with HT-DNA or recombinant IFNB1 +/− RU.521, followed by luciferase assay to measure type 1 interferon activation (b). A cytotoxicity dose response curve in THP-1 cells was performed across indicated RU.521 concentrations to calculate LD₅₀ (LD₅₀ = 31.4 µM) (c). The minimum numbers of biological and technical replicates for the assays were two and three, respectively (n = 6). Error bars represent SEM. Asterisks (*) denote P ≤ 0.05 where an unpaired t-test with Welch’s correction was used to compare the results.