. 2020 May 5;10:7596. doi: 10.1038/s41598-020-64265-0

Table 2.

Mass spectrometric parameters for quantitation.

Modification	Retention time (min)	Precursor		Product ion 1^a			Product ion 2^b			Product ion 3^b
Modification	Retention time (min)	m/z (amu)	DP (V)	m/z (amu)	CE (eV)	CXP (V)	m/z (amu)	CE (eV)	CXP (V)	m/z (amu)	CE (eV)	CXP (V)
Acylation
N⁶-formyl lysine	11.5	175.1	40	112.1	20	13	84.1	35	7	129.1	15	13
N⁶-malonyl lysine	13.4	233.2	45	126.2	20	10	84.2	38	10	170.3	22	12
N⁶-acetyl lysine	14.7	189.2	40	126.1	18	10	84.2	31	5	143.1	14	10
N⁶-succinyl lysine	16.8	247.1	50	84.3	40	10	184.4	21	12	130.1	26	12
N⁶-propionyl lysine	19.1	203.0	45	84.3	35	12	140.4	19	13	157.4	15	10
N⁶-butyryl lysine	23.3	217.0	50	84.3	32	5	154.3	18	10	171.4	17	10
Glycation
N⁶-glycoloyl lysine	10.4	205.2	40	142.1	20	11	84.1	36	14	56.2	64	8
N⁶-carboxymethyl lysine	9.6	205.1	42	130.2	17	9	84.1	30	14	56.1	59	9
N⁶-lactoyl lysine	14.3	219.2	40	156.2	20	8	84.1	35	9	173.1	17	8
N⁶-carboxyethyl lysine	14.4	219.1	54	84.1	33	7	130.1	18	11	56.1	59	8
glyoxal hydroimidazolone	17.3	215.1	48	100.1	20	8	70.1	38	11	116.2	20	10
methylglyoxal hydroimidazolone	23.0	229.2	45	114.2	21	9	70.2	45	11	116.1	21	9
furosine	24.4	255.2	50	84.1	33	7	130.3	21	10	192.0	23	12
Oxidative Stress
methionine sulfone	4.4	182.2	45	136.0	16	9	56.2	35	8	—	—	—
methionine sulfoxide	4.5	165.9	33	74.2	20	12	56.1	30	10	102.1	19	18
N⁶-glyoxylyl lysine	10.4	206.2	40	143.1	20	11	84.1	36	14	160.1	15	13
N⁶-pyruvoyl lysine	14.3	220.2	40	157.2	20	8	84.1	35	9	174.1	30	15
o-tyrosine	25.3	182.1	45	136.2	18	11	91.1	42	15	119.1	27	10
o,o-dityrosine	27.9	361.2	25	315.3	23	7	254.2	33	15	237.2	34	21
Citrullination
citrulline	5.5	176.2	40	159.2	14	11	70.3	35	11	113.1	22	7

^aMRM transition used for quantitation. ^bMRM transition used for confirmation.