Table 3.
Limits of detection (LOD) and limits of quantitation (LOQ).
| Modifications | [µmol/mol leucine equivalent] | |||||
|---|---|---|---|---|---|---|
| Histones | Mitochondria | Cytosol | ||||
| LOD | LOQ | LOD | LOQ | LOD | LOQ | |
| N6-formyl lysine | 1.1 | 3.8 | 1.8 | 6.0 | 2.5 | 8.3 |
| N6-acetyl lysine | 0.1 | 0.4 | 0.5 | 1.8 | 0.7 | 2.3 |
| N6-propionyl lysine | 0.1 | 0.4 | 0.2 | 0.6 | 0.1 | 0.4 |
| N6-butyryl lysine | 0.04 | 0.1 | 0.02 | 0.07 | 0.02 | 0.07 |
| N6-malonyl lysine | 0.9 | 2.9 | 0.3 | 0.9 | 0.4 | 1.3 |
| N6-succinyl lysine | 0.4 | 1.4 | 0.03 | 0.1 | 0.04 | 0.1 |
| CML | 0.4 | 1.2 | 0.5 | 1.7 | 0.7 | 2.4 |
| GALA | 0.3 | 0.9 | 0.1 | 0.3 | 0.1 | 0.2 |
| G-H3 | 0.6 | 2.0 | 1.0 | 3.4 | 1.1 | 3.6 |
| CEL | 0.4 | 1.3 | 0.5 | 1.8 | 0.5 | 1.8 |
| N6-lactoyl lysine | 0.3 | 1.0 | 0.1 | 0.2 | 0.03 | 0.1 |
| MG-H | 0.7 | 2.4 | 0.3 | 1.1 | 0.6 | 1.9 |
| furosine | 0.1 | 0.4 | 0.04 | 0.1 | 0.05 | 0.2 |
| N6-glyoxylyl lysine | 0.1 | 0.4 | 0.3 | 0.8 | 0.4 | 1.5 |
| N6-pyruvoyl lysine | 0.1 | 0.3 | 0.3 | 0.9 | 0.4 | 1.4 |
| o-tyrosine | 0.1 | 0.2 | 0.2 | 0.6 | 0.1 | 0.4 |
| o,o-dityrosine | 0.4 | 1.4 | 0.4 | 1.3 | 0.5 | 1.7 |
| methionine sulfoxide | 0.1 | 0.2 | 0.2 | 0.7 | 0.2 | 0.8 |
| methionine sulfone | 0.6 | 2.0 | 0.6 | 2.2 | 2.8 | 9.3 |
| citrulline | 0.4 | 1.3 | 0.4 | 1.2 | 0.3 | 0.9 |
CML = N6-carboxymethyl lysine; GALA = N6-glycoloyl lysine, G-H3 = glyoxal hydroimidazolone 3; CEL = N6-carboxyethyl lysine; MG-H = methylglyoxal hydroimidazolone.