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. 2020 May 5;3:210. doi: 10.1038/s42003-020-0893-5

Fig. 1. Srsf2 deletion resulted in HCC development in the aged HKO mice.

Fig. 1

a Survival curve analysis of Srsf2f/f and HKO mice under the general nursing or meticulous nursing. b Comparison of body weight between Srsf2f/f and HKO mice at indicated months (M) of age (n = 5). c, d Comparison of ratios of liver weight (LW) or spleen weight (SW) to body weight (BW) between Srsf2f/f and HKO mice at indicated ages (n = 4). e Representative livers of Srsf2f/f and HKO mice at 12M of age. f Representative hematoxylin/eosin (HE)-stained liver sections from Srsf2f/f (scale bars, 50 μm) and HKO mice with tumors (scale bars, 500 μm) at 12M (n = 3). g, h Enlarged HE staining of HKO mice with tumors shown in (f) (scale bars, 50 μm). i Representative immunostaining of Afp, Gs, Ki67, or P62 of liver sections (scale bars, 50 μm) from 12M Srsf2f/f and tumors of 12M mutant mice (n = 3). j Sirius-Red staining (scale bars, 100 μm) and CD45 staining (scale bars, 50 μm) of Srsf2f/f livers and non-tumorous areas of 12M mutant mice (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001 vs. matched control by Student’s t test (b, c, d).