Table 3.
Differently expressed proteins identified in seminal plasma from men with primary infertility compared with control men.
| Protein | Accession number | Average SC | Abundance | NSAF ratio | T-Test | Expression | ||
|---|---|---|---|---|---|---|---|---|
| Control | PI | Control | PI | PI/ Control | P-value | |||
| Semenogelin-1 preproprotein | 4506883 | 698.3 | 312.0 | H | H | 0.28 | 0.001 | UE |
| Semenogelin-2 precursor | 4506885 | 1321.3 | 598.0 | H | H | 0.30 | <0.001 | UE |
| Extracellular matrix protein 1 isoform 3 precursor | 322302700 | 66.0 | 80.3 | H | H | 0.63 | 0.001 | UE |
| Prolactin-inducible protein precursor | 4505821 | 258.3 | 277.3 | H | H | 0.66 | 0.002 | UE |
| Actin. Cytoplasmic 2 | 316659409 | 53.7 | 113.3 | M | H | 1.72 | 0.004 | OE |
| Triosephosphate isomerase isoform 2 | 226529917 | 15.0 | 43.7 | L | M | 2.08 | 0.012 | OE |
| Sorbitol dehydrogenase | 156627571 | 19.7 | 47.3 | L | M | 2.10 | 0.008 | OE |
| Transmembrane protease serine 2 isoform 1 | 227499990 | 8.0 | 23.0 | L | M | 2.13 | 0.001 | OE |
| Neprilysin isoform X1 | 578807443 | 15.0 | 63.7 | L | M | 2.18 | 0.008 | OE |
| Phosphoglycerate kinase 1 | 4505763 | 9.3 | 25.0 | L | M | 2.19 | 0.002 | OE |
| Prostaglandin-H2 D-isomerase precursor | 32171249 | 14.0 | 37.0 | L | M | 2.19 | 0.013 | OE |
| Serotransferrin precursor | 4557871 | 63.3 | 282.0 | M | H | 2.24 | <0.001 | OE |
| Peroxiredoxin-2 | 32189392 | 11.3 | 35.7 | L | M | 2.34 | 0.026 | OE |
| Sialate O-acetylesterase isoform 1 precursor | 24850115 | 6.7 | 28.0 | VL | M | 2.38 | 0.034 | OE |
| L-lactate dehydrogenase B chain | 291575128 | 9.0 | 23.7 | L | M | 2.39 | 0.011 | OE |
| Iggfc-binding protein precursor | 154146262 | 16.3 | 54.0 | L | M | 2.41 | 0.004 | OE |
| Serpin B6 isoform a | 41152086 | 8.0 | 23.7 | L | M | 2.41 | 0.004 | OE |
| Chloride intracellular channel protein 1 | 14251209 | 3.3 | 11.7 | VL | L | 2.50 | 0.001 | OE |
| Ras-related protein Rab-3B | 19923750 | 11.0 | 39.0 | L | M | 2.77 | 0.012 | OE |
| Creatine kinase B-type | 21536286 | 15.7 | 57.3 | L | M | 2.80 | 0.005 | OE |
| Di-N-acetylchitobiase precursor | 4758092 | 3.0 | 11.7 | VL | L | 3.28 | 0.003 | OE |
| CD63 antigen isoform A | 383872447 | 3.0 | 10.3 | VL | L | 3.30 | 0.005 | OE |
| Annexin A1 | 4502101 | 7.7 | 34.3 | VL | M | 3.37 | 0.015 | OE |
| L-lactate dehydrogenase A chain isoform 1 | 5031857 | 5.0 | 19.3 | VL | L | 3.37 | 0.010 | OE |
| Cytosolic non-specific dipeptidase isoform X2 | 530414265 | 8.7 | 37.3 | L | M | 3.47 | 0.011 | OE |
| Receptor-type tyrosine-protein phosphatase S isoform X7 | 530425347 | 5.0 | 25.0 | VL | M | 3.66 | 0.003 | OE |
| Annexin A2 isoform 2 | 50845386 | 4.7 | 22.0 | VL | M | 3.86 | 0.016 | OE |
| Ras-related protein Rab-27B isoform X1 | 530414276 | 2.0 | 10.0 | VL | L | 3.87 | 0.009 | OE |
| Protein-glutamine gamma-glutamyltransferase 4 | 156627577 | 31.3 | 255.3 | L | H | 4.10 | <0.001 | OE |
| Collagen alpha-1(XVIII) chain isoform 2 precursor | 110611233 | 1.3 | 9.0 | VL | L | 4.35 | 0.001 | OE |
| Plastin-2 isoform X2 | 530402335 | 10.0 | 62.0 | VL | M | 4.46 | 0.027 | OE |
| Desmocollin-1 isoform Dsc1a preproprotein | 13435361 | 2.0 | 11.3 | VL | L | 5.08 | 0.002 | OE |
| Laminin subunit gamma-1 precursor | 145309326 | 2.0 | 19.7 | VL | L | 5.26 | 0.006 | OE |
| Ras-related protein Rab-27A | 19923264 | 3.0 | 20.7 | VL | M | 5.58 | 0.001 | OE |
| Dipeptidase 3 isoform a precursor | 193211608 | 0.7 | 9.3 | VL | L | 6.84 | 0.009 | OE |
| Desmoplakin isoform I | 58530840 | 9.7 | 56.3 | L | M | 7.52 | 0.048 | OE |
| Agrin precursor | 54873613 | 1.3 | 15.0 | VL | L | 7.72 | 0.005 | OE |
| Amiloride-sensitive amine oxidase [copper-containing] isoform X3 | 578814090 | 2.7 | 40.7 | VL | M | 7.94 | 0.009 | OE |
| Laminin subunit alpha-5 isoform X1 | 578835999 | 1.7 | 27.0 | VL | M | 8.95 | 0.001 | OE |
| Lactoylglutathione lyase | 118402586 | 1.3 | 13.7 | VL | L | 8.96 | 0.001 | OE |
| Golgi apparatus protein 1 isoform 2 precursor | 224586815 | 0.7 | 8.7 | VL | L | 11.35 | 0.002 | OE |
| Choline transporter-like protein 4 isoform 1 | 148612887 | 0.3 | 8.7 | VL | L | 12.95 | 0.001 | OE |
| Junction plakoglobin isoform X1 | 530412116 | 2.0 | 32.7 | VL | M | 21.31 | 0.010 | OE |
| Polymeric immunoglobulin receptor isoform X1 | 530366266 | 0.3 | 24.0 | VL | M | 41.94 | 0.001 | OE |
| Cell division control protein 42 homolog isoform 1 precursor | 4757952 | 0.0 | 3.3 | — | VL | — | <0.001 | Unique |
| Glyoxalase domain-containing protein 4 | 217330598 | 0.0 | 2.3 | — | VL | — | <0.001 | Unique |
| Ferritin heavy chain | 56682959 | 0.0 | 4.0 | — | VL | — | <0.001 | Unique |
| Importin-5 isoform X2 | 530423350 | 0.0 | 5.0 | — | VL | — | 0.001 | Unique |
Abbreviations: SC - Spectral counts; NSAF - Normalized spectral abundance factor; H - High; M - Medium; L - Low; VL - Very Low; UE - Underexpressed; OE – Overexpressed; PI- Primary Infertility.