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. 2020 May 5;10:7536. doi: 10.1038/s41598-020-64416-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Brain energy metabolites and antioxidants and their correlations. (A) Brain metabolites in shams (black) and bile duct ligated (BDL) (grey) rats during the progression of the disease, expressed in absolute concentrations. (B) % difference in metabolite concentration between BDL rats and shams at each time point. Left y-axis: % change of the metabolite, right y-axis represents corresponding mmol/kgww (kg of wet weight) change. (C) Correlations of lactate and ascorbate. *Comparison between shams and BDL for each time-point; *(grey) significance compared to week 2; + significance compared to week 4.